Search results for " Real time PCR"

showing 6 items of 6 documents

Rapid, cost-effective, sensitive and quantitative detection of Acinetobacter baumannii from pneumonia patients

2011

Background and Objectives: Pneumonia with Acinetobacter baumannii has a major therapeutic problem in health care settings. Decision to initiate correct antibiotic therapy requires rapid identification and quantification of organism. The aim of this study was to develop a rapid and sensitive method for direct detection of A. baumannii from respiratory specimens. Materials and Methods: A Taqman real time PCR based on the sequence of blaoxa-51 was designed and used for direct detection of A. baumannii from 361 respiratory specimens of patients with pneumonia. All specimens were checked by conventional bacteriology in parallel. Results: The new real time PCR could detect less than 200 cfu per m…

Acinetobacter baumanniiReal time PCRSettore MED/07 - Microbiologia E Microbiologia Clinicalcsh:QR1-502VAPHAPPneumoniaHAP VAP Acinetobacter baumannii Real time PCR Pneumonialcsh:Microbiology
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Application of fnbA gene as new target for the species-specific and quantitative detection of Staphylococcus aureus directly from lower respiratory t…

2013

Staphylococcus aureus is a significant cause of hospital-acquired pneumonia (HAP), particularly in mechanically ventilated patients. We used the fibronectin-binding protein A gene (fnbA) for the species-specific and quantitative detection of S. aureus directly from lower respiratory tract (LRT) specimens by a Taq Man real time PCR. For this reason, a total of 269 lower respiratory tract (LRT) specimens collected from patients with hospital-acquired pneumonia were assayed. Amplification of fnbA in serial dilutions ranged from 10(9) CFU/ ml to 10(2) CFU/ml. Standard curve of triplicate every dilution had slope 3.34±0.1 and R2>0.99 with SD 0.1. Based on these data, the sensitivity and specif…

Microbiology (medical)fnbA Gene real time PCR respiratory infection Staphylococcus aureusSettore MED/07 - Microbiologia E Microbiologia ClinicaStaphylococcus aureusSerial dilutionRespiratory Systemlcsh:QR1-502medicine.disease_causeReal-Time Polymerase Chain ReactionSensitivity and SpecificityfnbA Genelcsh:MicrobiologyPathology and Forensic MedicineMicrobiologyrespiratory infectionPneumonia StaphylococcalmedicineTaqManlcsh:PathologyHumansAdhesins BacterialCross InfectionbiologyStaphylococcus. aureusRespiratory infectionGeneral Medicinemedicine.diseasePneumoniareal time PCRmedicine.anatomical_structureReal-time polymerase chain reactionMolecular Diagnostic TechniquesStaphylococcus aureusbiology.proteinProtein ARespiratory tractlcsh:RB1-214Indian journal of pathologymicrobiology
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AUTOPHAGY AND APOPTOSIS MODULATION BY AQUEOUS EXTRACTS FROM LEAVES AND RHIZOMES OF Posidonia oceanica ON HEPG2 HEPATOCARCINOMA CELLS

2023

Settore CHIM/10 - Chimica Degli Alimenticell biology caspases LC3 Beclin-1 p62/SQSTM1 hsp60 BCL2 BAX BAD FOS JUN DAPK western blot flow cytometry real time PCR acidic vesicular organelles annexin bindingSettore BIO/05 - ZoologiaSettore BIO/06 - Anatomia Comparata E Citologia
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Single tube real time PCR for detection of Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydophila pneumoniae and Legionella pneumophila from …

2012

We designed a multiplex real time PCR for rapid, sensitive and specific detection of Streptococcus pneumoniae, Legionella pneumophila, Chlamydophila pneumoniae and Mycoplasma pneumoniae. The study cases consisted of 129 patients with community acquired pneumonia (CAP). Bacteriological techniques were implemented for detection of the cultivable organisms. DNA were extracted from sputa, throat swabs, bronchoalveolar lavages and tracheal aspirates and used as templates in real time PCR. The primers and probes were designed for cbpA (S. pneumoniae), p1adhesin (M. pneumoniae), mip (L. pneumophila) and ompA (C. pneumoniae). After optimization of real time PCR for every organism, the experiments w…

Settore MED/07 - Microbiologia E Microbiologia ClinicaMycoplasma pneumoniaemedicine.disease_causeReal-Time Polymerase Chain ReactionLegionella pneumophilaSensitivity and SpecificityMicrobiologyLegionella pneumophilaCommunity-acquired pneumoniacommunity acquired pneumonia CAP real time PCR Streptococcus pneumonia Legionella pneumophila Chlamydophila pneumonia Mycoplasma pneumoniaeStreptococcus pneumoniaeMultiplex polymerase chain reactionmedicinePneumonia BacterialHumansMultiplexGeneral Immunology and MicrobiologybiologyBacteriaGeneral MedicineChlamydophila pneumoniaebiology.organism_classificationmedicine.diseaseVirologyrespiratory tract diseasesMycoplasma pneumoniaeCommunity-Acquired InfectionsReal-time polymerase chain reactionStreptococcus pneumoniaeChlamydophila pneumoniaeMultiplex Polymerase Chain ReactionActa microbiologica et immunologica Hungarica
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Coxiella burnetii spread in Sicily

2015

Q fever is a zoonotic disease caused by C. burnetii. Common reservoirs of this worldwide disease are wild and domestic animals, especially sheep, goats, cattle. Q fever has been considered also an occupational disease for abattoir workers, sheep shearers, livestock farmers, and veterinarians due to their direct contact with potentially infected animals. The aims of this study were to estimate C. burnetii spread in Sicilian livestock and among rangers that live and work in Western Sicily. ELISA test on animal serum and IFI test on seasonal rangers sera were carried out. Real Time PCR was performed on milk and vaginal swab samples collected from animals to search for the C. burnetii DNA. The …

Settore MED/44 - Medicina Del LavoroCoxiella burnetii seroprevalence Real Time PCR
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Quantification and viability assays of Toxoplasma gondii in commercial "Serrano" ham samples using magnetic capture real-time qPCR and bioassay techn…

2014

"Serrano" ham is a typical pork product from the Mediterranean area, highly valued for its flavour. To make Serrano ham, pork undergoes a salting and a subsequent fermentation process known as curing. Certain pigs used for meat production are an important source of Toxoplasma gondii infection in humans. We have developed a method for quantifying and assaying the viability of the T. gondii present in commercial Serrano ham samples. A magnetic capture method for the isolation of T. gondii DNA and a qRT-PCR were used to estimate the T. gondii burden in 475 commercial samples of "Serrano" ham in two presentation formats: ham pieces and sliced ham. The infectivity capacity of T. gondii in positi…

SwineFood ContaminationReal-Time Polymerase Chain ReactionMicrobiologyMagneticsMiceparasitic diseasesBioassayAnimalsHumansFood scienceInfectivityMice Inbred BALB CbiologySaltingToxoplasma gondiibiology.organism_classificationVirologyMeat ProductsQuantitative Real Time PCRToxoplasmosis AnimalSpainMediterranean areaBiological AssayToxoplasmaFood ScienceFood microbiology
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